Article
Gene transfer between related bacteria by electrotransformation: mapping Salmonella typhi genes in Salmonella typhimurium.
Journal of bacteriology - 1 Sept 1998
Toro C S, Mora G C, Figueroa-Bossi N
Abstract excerpt
Transfer of newly isolated mutations into a fresh background is an essential step of genetic analysis and strain construction. Gene transfer is hampered in Salmonella typhi and in other pathogenic bacteria by the lack of a generalized transduction system. We show here that this problem can be partially circumvented by using electrotransformation as a means for delivering S. typhi DNA into suitable S. typhi or...
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