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Article

Protein electroporation into Chlamydomonas for mutant rescue.

Methods in cell biology - 1 Jan 2009

Hayashi Masahito, Kamiya Ritsu

Abstract excerpt

Electroporation can be used to deliver proteins into Chlamydomonas cells. This technique is particularly useful to demonstrate rescue of paralyzed-flagella mutants with recombinant proteins that complement the mutations. The appearance of motile cells immediately provides assurance that the recombinant proteins have their native structures and are functional. It can be also used for the introduction of...

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PMID: 20409801
DOI: 10.1016/S0091-679X(08)92007-0

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