Article
Prediction of the evolution of ceftazidime resistance in extended-spectrum beta-lactamase CTX-M-9.
Antimicrobial agents and chemotherapy - 1 Feb 2006
Delmas J, Robin F, Carvalho F, Mongaret C, Bonnet R
Abstract excerpt
A random mutagenesis technique was used to predict the evolutionary potential of beta-lactamase CTX-M-9 toward the acquisition of improved catalytic activity against ceftazidime. Thirty CTX-M mutants were obtained during three rounds of mutagenesis. These mutants conferred 1- to 128-fold-higher MICs of ceftazidime than the parental enzyme CTX-M-9. The CTX-M mutants contained one to six amino acid substitutions....
Topics
- Ceftazidime
- Cephalosporin Resistance
- Escherichia coli
- Escherichia coli Proteins
- Microbial Sensitivity Tests
- Mutation
- Sequence Analysis, DNA
- Structure-Activity Relationship
- beta-Lactamases
- beta-Lactams