Article
Iron-responsive degradation of iron-regulatory protein 1 does not require the Fe-S cluster.
The EMBO journal - 8 Feb 2006
Clarke Stephen L, Vasanthakumar Aparna, Anderson Sheila A, Pondarré Corinne, Koh Cheryl M, Deck Kathryn M, Pitula Joseph S, Epstein Charles J, Fleming Mark D, Eisenstein Richard S
Abstract excerpt
The generally accepted role of iron-regulatory protein 1 (IRP1) in orchestrating the fate of iron-regulated mRNAs depends on the interconversion of its cytosolic aconitase and RNA-binding forms through assembly/disassembly of its Fe-S cluster, without altering protein abundance. Here, we show that IRP1 protein abundance can be iron-regulated. Modulation of IRP1 abundance by iron did not require assembly of the...
Topics
- Animals
- Cell Line
- Hemin
- Humans
- Iron
- Iron Regulatory Protein 1
- Mice
- Mice, Knockout
- Mutation
- Phosphorylation
- RNA, Messenger
- RNA-Binding Proteins
- Serine