Article
Structural determinants of slippage-mediated mutations by human immunodeficiency virus type 1 reverse transcriptase.
The Journal of biological chemistry - 17 Mar 2006
Hamburgh Monica E, Curr Kenneth A, Monaghan Melissa, Rao Vasudev R, Tripathi Snehlata, Preston Bradley D, Sarafianos Stefan, Arnold Eddy, Darden Thomas, Prasad Vinayaka R
Abstract excerpt
Single-base deletions at nucleotide runs or -1 frameshifting by human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) result from template slippage during polymerization. In crystal structures of HIV-1 RT complexed with DNA-DNA template-primer, the palm subdomain in the template cleft contacts the template backbone near the proposed site of slippage via the Glu(89) side chain. We investigated the...
Topics
- Base Sequence
- DNA
- DNA Primers
- DNA, Viral
- Drug Resistance, Viral
- Frameshift Mutation
- Gene Deletion
- Genes, Viral
- Glutamic Acid
- HIV Reverse Transcriptase
- HIV-1
- Lac Operon
- Lysine