Article
Mutational analysis of the fingers and palm subdomains of human immunodeficiency virus type-1 (HIV-1) reverse transcriptase.
Journal of molecular biology - 28 Oct 1994
Boyer P L, Ferris A L, Clark P, Whitmer J, Frank P, Tantillo C, Arnold E, Hughes S H
Abstract excerpt
We have analyzed the human immunodeficiency virus type-1 reverse transcriptase (HIV-1 RT) polymerase domain between amino acids 91 and 157 by site-directed mutagenesis. We have constructed a series of amino acid substitutions using BspMI cassettes, and have assayed the RNA-dependent DNA polymeras...
Topics
- Amino Acids
- Base Sequence
- DNA Mutational Analysis
- DNA-Directed DNA Polymerase
- HIV Reverse Transcriptase
- HIV-1
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Polydeoxyribonucleotides
- Protein Binding
- Protein Conformation
- RNA-Directed DNA Polymerase
- Ribonuclease H