Substitutions at Phe61 in the beta3-beta4 hairpin of HIV-1 reverse transcriptase reveal a role for the Fingers subdomain in strand displacement DNA synthesis.

Abstract excerpt

Unlike most DNA polymerases, retroviral reverse transcriptases (RTs) are capable of strand displacement DNA synthesis in vitro, unassisted by other proteins. While human immunodeficiency virus type 1 (HIV-1) RT has been shown to possess this rare ability, the structural determinants responsible are unknown. X-Ray crystallographic and biochemical studies have indicated that the beta3-beta4 hairpin of the fingers...

Topics

• Amino Acid Substitution
• Animals
• Base Sequence
• DNA Replication
• DNA, Viral
• HIV Reverse Transcriptase
• Humans
• Models, Molecular
• Molecular Sequence Data
• Nucleic Acid Conformation
• Phenotype
• Phenylalanine
• Protein Structure, Secondary
• Protein Structure, Tertiary