Article
Steady-state and time-resolved fluorescence studies of the intestinal fatty acid binding protein.
Proteins - 1 May 2006
Chattopadhyay Krishnananda, Frieden Carl
Abstract excerpt
The intestinal fatty acid binding protein contains two tryptophan residues (Trp6 and Trp82) both of which have been shown by X-ray and NMR methods to be buried in hydrophobic clusters. By using a combination of steady-state and time-resolved fluorescence experiments, we have deconvoluted the lifetime weighted contribution of each of the tryptophans to the steady-state fluorescence quantum yield. While Trp82 has...
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