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Article

Selection of primer-template sequences that bind human immunodeficiency virus reverse transcriptase with high affinity

8 Jan 2006

Abstract excerpt

A SELEX (systematic evolution of ligands by exponential enrichment)-based approach was developed to determine whether HIV-RT showed preference for particular primer-template sequences. A 70 nt duplex DNA was designed with 20 nt fixed flanking sequences at the 3' and 5' ends and a randomized 30 nt internal sequence. The fixed sequence at the 5' end contained a BbsI site six bases removed from the randomized...

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PMID: 16397296
DOI: 10.1093/nar/gkj426

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