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Article

Determination of the termination efficiency of the transcription terminator using different fluorescent profiles in green fluorescent protein mutants.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Dec 2005

Nojima Takahiko, Lin Angela C, Fujii Teruo, Endo Isao

Abstract excerpt

An approach in determining the intrinsic termination efficiency (%T) of transcription termination using green fluorescent protein (GFP) mutants was developed. This approach utilizes a cassette vector in which the tested terminator is introduced between two GFP mutant genes: an ultraviolet-optimized mutant (GFPuv: F99S, M153T, V163A) and a blue-shifted mutant (BFP: F64L, S65T, T145F). The ratio of the fluorescence...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 16379390
DOI: 10.2116/analsci.21.1479

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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