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Article

An improved GFP cloning cassette designed for prokaryotic transcriptional fusions.

Gene - 3 Jun 1997

Miller W G, Lindow S E

Abstract excerpt

A new gfp cloning cassette designed for prokaryotic transcriptional fusions has been constructed. This cassette consists of gfp (containing the S65T 'red-shift' [Heim et al. (1995) Nature 373, 663-664] and F64L 'protein solubility' [Cormack et al. (1996) Gene 173, 33-38] mutations) flanked by con...

Topics

Cloning, Molecular
Escherichia coli
Fluorescence
Gene Expression
Genes, Bacterial
Genes, Reporter
Genetic Vectors
Green Fluorescent Proteins
Iron
Luminescent Proteins
Mutation
Promoter Regions, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Biosynthesis

Pseudomonas

Recombinant Fusion Proteins

Solubility

Transcription, Genetic

Source

Identifiers

PMID: 9218713
DOI: 10.1016/s0378-1119(97)00051-6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9218713-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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