Article
An improved GFP cloning cassette designed for prokaryotic transcriptional fusions.
Gene - 3 Jun 1997
Miller W G, Lindow S E
Abstract excerpt
A new gfp cloning cassette designed for prokaryotic transcriptional fusions has been constructed. This cassette consists of gfp (containing the S65T 'red-shift' [Heim et al. (1995) Nature 373, 663-664] and F64L 'protein solubility' [Cormack et al. (1996) Gene 173, 33-38] mutations) flanked by convenient restriction sites, a translational enhancer, and a consensus ribosome binding site with an optimized spacer...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
