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Article

Rapid and Simple Method for Detecting the Toxin B Gene of <i>Clostridium difficile</i> in Stool Specimens by Loop-Mediated Isothermal Amplification

1 Dec 2005

Abstract excerpt

We applied the loop-mediated isothermal amplification (LAMP) assay to the detection of the toxin B gene (tcdB) of Clostridium difficile for identification of toxin B (TcdB)-positive C. difficile strains and detection of tcdB in stool specimens. tcdB was detected in all toxin A (TcdA)-positive, TcdB-positive (A(+)B(+)) and TcdA-negative, TcdB-positive (A(-)B(+)) C. difficile strains but not from TcdA-negative,...

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PMID: 16333105
DOI: 10.1128/jcm.43.12.6108-6112.2005

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