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Multiplex PCR Targeting<i>tpi</i>(Triose Phosphate Isomerase),<i>tcdA</i>(Toxin A), and<i>tcdB</i>(Toxin B) Genes for Toxigenic Culture of<i>Clostridium difficile</i>

1 Dec 2004

Abstract excerpt

A multiplex PCR toxigenic culture approach was designed for simultaneous identification and toxigenic type characterization of Clostridium difficile isolates. Three pairs of primers were designed for the amplification of (i) a species-specific internal fragment of the tpi (triose phosphate isomerase) gene, (ii) an internal fragment of the tcdB (toxin B) gene, and (iii) an internal fragment of the tcdA (toxin A)...

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PMID: 15583303
DOI: 10.1128/jcm.42.12.5710-5714.2004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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