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Real-Time Multiplex Polymerase Chain Reaction Assay for Rapid Detection of <i>Clostridium difficile</i> Toxin-Encoding Strains

30 Jan 2010

Abstract excerpt

Clostridium difficile is considered an important emerging pathogen capable of causing disease in humans and animal species. In our study, we developed and evaluated a multiplex real-time polymerase chain reaction (PCR) assay for the detection of C. difficile genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA and cdtB). The standardized real-time PCR assay for toxin genes of C. difficile was...

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PMID: 20113206
DOI: 10.1089/fpd.2009.0483

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