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Development of TaqMan-Based Quantitative PCR for Sensitive and Selective Detection of Toxigenic Clostridium difficile in Human Stools

31 Oct 2014

Abstract excerpt

BACKGROUND: Clostridium difficile is the main cause of nosocomial diarrhea, but is also found in asymptomatic subjects that are potentially involved in transmission of C. difficile infection. A sensitive and accurate detection method of C. difficile, especially toxigenic strains is indispensable for the epidemiological investigation. METHODS: TaqMan-based quantitative-PCR (qPCR) method for targeting 16S rRNA,...

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PMID: 25360662
DOI: 10.1371/journal.pone.0111684

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