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Article

Structural comparison of the unstable drkN SH3 domain and a stable mutant.

Biochemistry - 29 Nov 2005

Bezsonova Irina, Singer Alex, Choy Wing-Yiu, Tollinger Martin, Forman-Kay Julie D

Abstract excerpt

The N-terminal SH3 domain of the Drosophila adapter protein Drk (drkN SH3 domain) is marginally stable (DeltaG(U) = 1 kcal/mol) and exists in equilibrium between folded and highly populated unfolded states. The single substitution T22G, however, completely stabilizes the protein (DeltaG(U) = 4.0 kcal/mol). To probe the causes of instability of the wild-type (WT) protein and the dramatic stabilization of the...

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Identifiers

PMID: 16300404
DOI: 10.1021/bi0512795

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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