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Article

Unusual effects of an engineered disulfide on global and local protein stability.

Biochemistry - 11 Jun 1996

Betz S F, Marmorino J L, Saunders A J, Doyle D F, Young G B, Pielak G J

Abstract excerpt

The global and local stabilities of a eukaryotic ferricytochrome c variant with an engineered disulfide are examined. The disulfide connects position 20, which is usually a valine, to position 102, which is usually a threonine. The cross-linked variant is approximately 1.2 kcal mol-1 less stable than the wild-type protein at 298 K, pH 4.6, in H2O and D2O. Circular dichroism studies show that the decreased...

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Identifiers

PMID: 8652519
DOI: 10.1021/bi9528558

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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