Article
Mutation discovery in bacterial genomes: metronidazole resistance in Helicobacter pylori.
Nature methods - 1 Dec 2005
Albert Thomas J, Dailidiene Daiva, Dailide Giedrius, Norton Jason E, Kalia Awdhesh, Richmond Todd A, Molla Michael, Singh Jaz, Green Roland D, Berg Douglas E
Abstract excerpt
We developed a microarray hybridization-based method, 'comparative genome sequencing' (CGS), to find mutations in bacterial genomes and used it to study metronidazole resistance in H. pylori. CGS identified mutations in several genes, most likely affecting metronidazole activation, and produced no false positives in analysis of three megabases. We conclude that CGS identifies mutations in bacterial genomes...
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