Article
Deletion of the membrane anchoring region of tissue factor abolishes autoactivation of factor VII but not cofactor function. Analysis of a mutant with a selective deficiency in activity.
The Journal of biological chemistry - 15 Jul 1992
Neuenschwander P F, Morrissey J H
Abstract excerpt
The activation of human blood coagulation factor VII can occur by the feedback activity of either factor VIIa (autoactivation) or factor Xa. Both of these reactions are known to be enhanced by the presence of tissue factor, an integral membrane protein and the cofactor for factor VIIa. We examine here the activation of 125I-factor VII by both factor VIIa and factor Xa employing a mutant soluble form of tissue...
Topics
- Cell Line
- Chromatography, Affinity
- Chromatography, Ion Exchange
- Chromosome Deletion
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Factor VII
- Factor VIIa
- Factor Xa
- Feedback