Article
Mutation of a single conserved nucleotide between the cloverleaf and internal ribosome entry site attenuates poliovirus neurovirulence.
Journal of virology - 1 Nov 2005
De Jesus Nidia, Franco David, Paul Aniko, Wimmer Eckard, Cello Jeronimo
Abstract excerpt
The chemical synthesis of poliovirus (PV) cDNA combined with the cell-free synthesis of infectious particles yielded virus whose mouse neurovirulence was highly attenuated (J. Cello, A. V. Paul, and E. Wimmer, Science 297:1016-1018, 2002). Compared to the wild-type PV1 (Mahoney) [PV1(M)] sequence...
Topics
- Animals
- Base Sequence
- Conserved Sequence
- DNA, Complementary
- DNA, Viral
- Mice
- Mutation
- Poliovirus
- Polymorphism, Single Nucleotide
- Protein Biosynthesis
- RNA, Viral
- Ribosomes
- Transcription, Genetic
- Transfection
- Virulence