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Article

Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli.

Journal of bacteriology - 1 Jul 1992

Guillon J M, Mechulam Y, Schmitter J M, Blanquet S, Fayat G

Abstract excerpt

In bacteria, as well as in chloroplasts and mitochondria, the free amino group of the methionylated initiator tRNA(fMet) is specifically modified by the addition of a formyl group. The importance of this modification remains unclear. With the availability of pure Escherichia coli 10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase, the enzyme catalyzing Met-tRNA(fMet) formylation, the...

Topics

Acyltransferases
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Escherichia coli
Gene Library
Genes, Bacterial
Genotype
Hydroxymethyl and Formyl Transferases
Molecular Sequence Data
Oligodeoxyribonucleotides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligonucleotide Probes

Plasmids

Promoter Regions, Genetic

Restriction Mapping

Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 1624424
DOI: 10.1128/jb.174.13.4294-4301.1992
PMCID: PMC206212

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1624424-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli.PMID 1624424Read ↗

Nucleotides of tRNA governing the specificity of Escherichia coli methionyl-tRNA(fMet) formyltransferase.Semantic proximity Read ↗

Crucial contribution of the multiple copies of the initiator tRNA genes in the fidelity of tRNA(fMet) selection on the ribosomal P-site in Escherichia coli.Semantic proximity Read ↗

Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase that compensate for the formylation defect of a mutant tRNA aminoacylated with lysine.Semantic proximity Read ↗

Characterization of the Thermus thermophilus locus encoding peptide deformylase and methionyl-tRNA(fMet) formyltransferase.Semantic proximity Read ↗

Mutations in three distinct loci cause resistance to peptide deformylase inhibitors in Bacillus subtilis.Semantic proximity Read ↗

Transcription and regulation of expression of the Escherichia coli methionyl-tRNA synthetase gene.Semantic proximity Read ↗

Formylation is not essential for initiation of protein synthesis in all eubacteria.Semantic proximity Read ↗

Expression of Escherichia coli methionyl-tRNA formyltransferase in Saccharomyces cerevisiae leads to formylation of the cytoplasmic initiator tRNA and possibly to initiation of protein synthesis with formylmethionine.Semantic proximity Read ↗

Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli.Semantic proximity Read ↗

Important role of the amino acid attached to tRNA in formylation and in initiation of protein synthesis in Escherichia coli.Semantic proximity Read ↗

Escherichia coli initiator tRNA: structure-function relationships and interactions with the translational machinery.Semantic proximity Read ↗

Reducing the fitness cost of antibiotic resistance by amplification of initiator tRNA genes.Semantic proximity Read ↗

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