Article
Important role of the amino acid attached to tRNA in formylation and in initiation of protein synthesis in Escherichia coli.
The Journal of biological chemistry - 12 Jan 1996
Li S, Kumar N V, Varshney U, RajBhandary U L
Abstract excerpt
In attempts to convert an elongator tRNA to an initiator tRNA, we previously generated a mutant elongator methionine tRNA carrying an anticodon sequence change from CAU to CUA along with the two features important for activity of Escherichia coli initiator tRNA in initiation. This mutant tRNA (Mi:2 tRNA) was active in initiation in vivo but only when aminoacylated with methionine by overproduction of...
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