Article
Nucleotides of tRNA governing the specificity of Escherichia coli methionyl-tRNA(fMet) formyltransferase.
Journal of molecular biology - 20 Mar 1992
Guillon J M, Meinnel T, Mechulam Y, Lazennec C, Blanquet S, Fayat G
Abstract excerpt
In Escherichia coli, the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) is specifically modified by a transformylation reaction. To identify the nucleotides governing the recognition of the tRNA substrate by the formylase, initiator tRNA(fMet) was changed into an elongator tRNA with the help of an in vivo selection method. All the mutations isolated were in the tRNA acceptor arm, at positions 72...
Topics
- Acyltransferases
- Anticodon
- Base Composition
- Base Sequence
- Escherichia coli
- Hydroxymethyl and Formyl Transferases
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Bacterial
- RNA, Transfer, Met