Expression of Escherichia coli methionyl-tRNA formyltransferase in Saccharomyces cerevisiae leads to formylation of the cytoplasmic initiator tRNA and possibly to initiation of protein synthesis with formylmethionine.

Abstract excerpt

Protein synthesis in eukaryotic cytoplasm and in archaebacteria is initiated with methionine, whereas, that in eubacteria and in eukaryotic organelles, such as mitochondria and chloroplasts, is initiated with formylmethionine. In view of this clear distinction, we have investigated whether protein synthesis in the eukaryotic cytoplasm can be initiated with formylmethionine, and, if so, what the consequences are...

Topics

• Amidohydrolases
• Aminopeptidases
• Bacterial Proteins
• Base Sequence
• Cell Division
• Cytoplasm
• Electrophoresis, Gel, Two-Dimensional
• Escherichia coli
• Formates
• Gene Expression Regulation, Fungal
• Hydroxymethyl and Formyl Transferases
• Molecular Sequence Data