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Article

Characterization of the dinuclear metal center of Pyrococcus furiosus prolidase by analysis of targeted mutants.

FEBS letters - 7 Nov 2005

Du Xuelian, Tove Sherry, Kast-Hutcheson Karen, Grunden Amy M

Abstract excerpt

Prolidases are dipeptidases specific for cleavage of Xaa-Pro dipeptides. Pyrococcus furiosus prolidase is a homodimer having one Co-bound dinuclear metal cluster per monomer with one tightly bound Co(II) site and the other loosely bound (Kd 0.24 mM). To identify which Co site is tight-binding and which is loose-binding, site-directed mutagenesis was used to modify amino acid residues that participate in binding...

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Identifiers

PMID: 16243319
DOI: 10.1016/j.febslet.2005.09.086

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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