Article
Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin.
Cell - 21 Oct 2005
Raisner Ryan M, Hartley Paul D, Meneghini Marc D, Bao Marie Z, Liu Chih Long, Schreiber Stuart L, Rando Oliver J, Madhani Hiten D
Abstract excerpt
In S. cerevisiae, histone variant H2A.Z is deposited in euchromatin at the flanks of silent heterochromatin to prevent its ectopic spread. We show that H2A.Z nucleosomes are found at promoter regions of nearly all genes in euchromatin. They generally occur as two positioned nucleosomes that flank a nucleosome-free region (NFR) that contains the transcription start site. Astonishingly, enrichment at 5' ends is...
Topics
- Acetylation
- Amino Acid Substitution
- Arginine
- Binding Sites
- Chromatin Immunoprecipitation
- Chromosome Mapping
- Chromosomes
- Codon, Initiator
- DNA, Fungal
- DNA, Intergenic
- DNA-Binding Proteins