Article
A prohormone convertase cleavage site within a predicted alpha-helix mediates sorting of the neuronal and endocrine polypeptide VGF into the regulated secretory pathway.
The Journal of biological chemistry - 16 Dec 2005
Garcia Angelo L, Han Shan-Kuo, Janssen William G, Khaing Zin Z, Ito Timothy, Glucksman Marc J, Benson Deanna L, Salton Stephen R J
Abstract excerpt
Distinct intracellular pathways are involved in regulated and constitutive protein secretion from neuronal and endocrine cells, yet the peptide signals and molecular mechanisms responsible for targeting and retention of soluble proteins in secretory granules are incompletely understood. By using confocal microscopy and subcellular fractionation, we examined trafficking of the neuronal and endocrine peptide...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Binding Sites
- Blotting, Western
- Cell Line
- Cell Membrane
- Centrifugation, Density Gradient
- Chromogranins
- Detergents