Article
In vivo directed evolution for thermostabilization of Escherichia coli hygromycin B phosphotransferase and the use of the gene as a selection marker in the host-vector system of Thermus thermophilus.
Journal of bioscience and bioengineering - 1 Aug 2005
Nakamura Akira, Takakura Yasuaki, Kobayashi Hideo, Hoshino Takayuki
Abstract excerpt
An in vivo-directed evolutionary strategy was used to obtain a thermostabilized Escherichia coli hygromycin B phosphotransferase, using a host-vector system of Thermus thermophilus. Introduction of the mutant gene containing two amino acid substitutions, S52T and W238C, which was previously reported by Cannio et al. [J. Bacteriol., 180, 3237-3240 (1998)], did not confer hygromycin resistance on T. thermophilus...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.