Article
Thermoadaptation of a mesophilic hygromycin B phosphotransferase by directed evolution in hyperthermophilic Archaea: selection of a stable genetic marker for DNA transfer into Sulfolobus solfataricus.
Extremophiles : life under extreme conditions - 1 Jun 2001
Cannio R, Contursi P, Rossi M, Bartolucci S
Abstract excerpt
A mutated version of the hygromycin B phosphotransferase (hph(mut)) gene from Escherichia coli, isolated by directed evolution at 75 degrees C in transformants of a thermophilic strain of Sulfolobus solfataricus, was characterized with respect to its genetic stability in both the original mesophilic and the new thermophilic hosts. This gene was demonstrated to be able to express the hygromycin B resistance...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.