Article
Comparison of terbium (III) luminescence enhancement in mutants of EF hand calcium binding proteins.
The Journal of biological chemistry - 5 Jul 1992
Hogue C W, MacManus J P, Banville D, Szabo A G
Abstract excerpt
The luminescent isomorphous Ca2+ analogue, Tb3+, can be bound in the 12-amino acid metal binding sites of proteins of the EF hand family, and its luminescence can be enhanced by energy transfer from a nearby aromatic amino acid. Tb3+ can be used as a sensitive luminescent probe of the structure a...
Topics
- Amino Acid Sequence
- Calcium-Binding Proteins
- Calmodulin
- Cations
- Luminescent Measurements
- Molecular Sequence Data
- Mutation
- Parvalbumins
- Spectrometry, Fluorescence
- Terbium