Characterization of Eu(III) binding to a series of calmodulin binding site mutants using laser-induced Eu(III) luminescence spectroscopy.

doi:10.1016/s0301-4622(96)02179-5

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Article

Characterization of Eu(III) binding to a series of calmodulin binding site mutants using laser-induced Eu(III) luminescence spectroscopy.

Biophysical chemistry - 10 Dec 1996

Bruno J, Horrocks W D, Beckingham K

Abstract excerpt

Laser-induced luminescence techniques were used in a rigorous evaluation of the Eu(3+)-binding behavior of a recombinant (Drosophila melanogaster) calmodulin and a series of calmodulin binding site mutants in which the bidentate glutamic acid residue in position 12 of each metal ion binding loop...

Topics

Animals
Binding Sites
Calcium
Calmodulin
Chemical Phenomena
Chemistry
Drosophila melanogaster
Europium
Lasers
Luminescent Measurements
Mutation
Protein Binding
Recombinant Proteins
Spectrum Analysis
Water

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