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Article

Metal-binding studies for a de novo designed calcium-binding protein.

Protein engineering - 1 Jul 2002

Wilkins Anna L, Ye Yiming, Yang Wei, Lee Hsiau-Wei, Liu Zhi-ren, Yang Jenny J

Abstract excerpt

To understand the key determinants in calcium-binding affinity, a calcium-binding site with pentagonal bipyramid geometry was designed into a non-calcium-binding protein, domain 1 of CD2. This metal-binding protein has five mutations with a net charge in the coordination sphere of -5 and is termed DEEEE. Fluorescence resonance energy transfer was used to determine the metal-binding affinity of DEEEE to the...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 12200539
DOI: 10.1093/protein/15.7.571

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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