Article
Triplex targeted genomic crosslinks enter separable deletion and base substitution pathways.
Nucleic acids research - 1 Jan 2005
Richards Sally, Liu Su-Ting, Majumdar Alokes, Liu Ji-Lan, Nairn Rodney S, Bernier Michel, Maher Veronica, Seidman Michael M
Abstract excerpt
We have synthesized triple helix forming oligonucleotides (TFOs) that target a psoralen (pso) interstrand crosslink to a specific chromosomal site in mammalian cells. Mutagenesis of the targeted crosslinks results in base substitutions and deletions. Identification of the gene products involved in mutation formation is important for developing practical applications of pso-TFOs, and may be informative about the...
Topics
- Animals
- Base Sequence
- CHO Cells
- Cricetinae
- Cricetulus
- Cross-Linking Reagents
- DNA
- DNA Repair
- DNA-Binding Proteins
- DNA-Directed DNA Polymerase
- Ficusin