Article
Genomic profiling maps loss of heterozygosity and defines the timing and stage dependence of epigenetic and genetic events in Wilms' tumors.
Molecular cancer research : MCR - 1 Sept 2005
Yuan Eric, Li Chi-Ming, Yamashiro Darrell J, Kandel Jessica, Thaker Harshwardhan, Murty Vundavalli V, Tycko Benjamin
Abstract excerpt
To understand genetic and epigenetic pathways in Wilms' tumors, we carried out a genome scan for loss of heterozygosity (LOH) using Affymetrix 10K single nucleotide polymorphism (SNP) chips and supplemented the data with karyotype information. To score loss of imprinting (LOI) of the IGF2 gene, we assessed DNA methylation of the H19 5' differentially methylated region (DMR). Few chromosomal regions other than...
Topics
- Blotting, Northern
- Blotting, Southern
- Chromosomes, Human
- DNA Fingerprinting
- DNA Methylation
- Epigenesis, Genetic
- Genome
- Humans
- Insulin-Like Growth Factor II
- Kidney Neoplasms
- Loss of Heterozygosity
- Microarray Analysis
- Neoplasm Recurrence, Local