Article
Efficient gene disruption in the koji-mold Aspergillus sojae using a novel variation of the positive-negative method.
Molecular genetics and genomics : MGG - 1 Oct 2004
Takahashi T, Hatamoto O, Koyama Y, Abe K
Abstract excerpt
When no phenotypic screen is available, gene disruption in the koji-mold Aspergillus sojae is a time-consuming process, owing to the low frequency of homologous recombination. To achieve efficient gene disruption in the koji-mold, we developed a novel positive-negative selection method to enrich for homologous recombinants. The pyrG gene from A. sojae was used as a positive selection marker for transformants, and...
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