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Article

Clinical applications of whole-blood PCR with real-time instrumentation.

Clinical chemistry - 1 Nov 2005

Castley Alison, Higgins Melinda, Ivey John, Mamotte Cyril, Sayer David C, Christiansen Frank T

Abstract excerpt

BACKGROUND: As the genetic basis of many human diseases is being discovered, there is increasing need for the detection of single-nucleotide polymorphisms/mutations in medical laboratories. We describe an innovative approach that combines PCR amplification directly on whole blood and real-time detection PCR technology (WB-RTD PCR). METHODS: We compared WB-RTD PCR with the method for extracted DNA-RTD PCR for the...

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PMID: 16166168
DOI: 10.1373/clinchem.2005.055327

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