Article
Evaluation of the Roche LightCycler: a simple and rapid method for direct detection of factor V Leiden and prothrombin G20210A genotypes from blood samples without the need for DNA extraction.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis - 1 Jul 2003
Cooper Peter C, Cooper Susan M, Smith Julie M, Kitchen Steven, Makris Michael
Abstract excerpt
The Roche LightCycler is a micro-volume thermocycler that combines extremely rapid polymerase chain reaction with fluorescence resonance energy transfer analysis of amplified products. We have evaluated the use of minimally processed blood samples for detection of two point mutations known to increase the risk of venous thromboembolism. Results from the LightCycler using the supernatant of diluted heated blood...
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