Article
Establishment of Tn5096-based transposon mutagenesis in Gordonia polyisoprenivorans.
Applied and environmental microbiology - 1 Sept 2005
Banh Quyen, Arenskötter Matthias, Steinbüchel Alexander
Abstract excerpt
The transposons Tn5, Tn10, Tn611, and Tn5096 were characterized regarding transposition in Gordonia polyisoprenivorans strain VH2. No insertional mutants were obtained employing Tn5 or Tn10. The thermosensitive plasmid pCG79 harboring Tn611 integrated into the chromosome of G. polyisoprenivorans; however, the insertional mutants were fairly unstable und reverted frequently to the wild-type phenotype. In contrast,...
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