Article
Transposon vectors for stable chromosomal integration of cloned genes in rhizosphere bacteria.
Gene - 1 Apr 1991
Kaniga K, Davison J
Abstract excerpt
A series of Tn5-based transposon-cloning vectors, in which many unique restriction sites lie within the transposon, have been constructed. These transposon vectors can be delivered, by conjugation, using a delivery vehicle containing a pBR322 replicon and the mobilization genes of plasmid RK2. In Pseudomonas sp., this delivery vehicle acts as a suicide plasmid, permitting transposition to the chromosome to be...
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