Article
Transposon vectors for stable chromosomal integration of cloned genes in rhizosphere bacteria.
Gene - 1 Apr 1991
Kaniga K, Davison J
Abstract excerpt
A series of Tn5-based transposon-cloning vectors, in which many unique restriction sites lie within the transposon, have been constructed. These transposon vectors can be delivered, by conjugation, using a delivery vehicle containing a pBR322 replicon and the mobilization genes of plasmid RK2. In...
Topics
- Blotting, Southern
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors
- Genotype
- Plasmids
- Pseudomonas
- Restriction Mapping