Article
Transfection of human 5-hydroxytryptamine1A receptors in NIH-3T3 fibroblasts: effects of increasing receptor density on the coupling of 5-hydroxytryptamine1A receptors to adenylyl cyclase.
Molecular pharmacology - 1 Jun 1992
Varrault A, Journot L, Audigier Y, Bockaert J
Abstract excerpt
Human serotonin [5-hydroxytryptamine (5-HT)1A] receptors have been transfected in NIH-3T3 cells, and their pharmacology and coupling to adenylyl cyclase have been analyzed. Three cellular preparations were used, 1) monoclonal cell lines (clones 6, 2B, and 4B), expressing 45, 280, and 500 fmol of 5-HT1A receptors/mg of protein, respectively; 2) clones 6, 2B, and 4B in which the concentration of 5-HT1A receptors...
Topics
- 3T3 Cells
- Adenylyl Cyclase Inhibitors
- Adenylyl Cyclases
- Animals
- Clone Cells
- Dexamethasone
- GTP-Binding Proteins
- Genetic Variation
- Humans
- Kinetics
- Mice