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Article

A protein engineering study of the role of aspartate 158 in the catalytic mechanism of papain.

Biochemistry - 17 Jul 1990

Ménard R, Khouri H E, Plouffe C, Dupras R, Ripoll D, Vernet T, Tessier D C, Lalberté F, Thomas D Y, Storer A C

Abstract excerpt

The controversy concerning the various suggested roles for the side chain of Asp158 in the active site of papain has been clarified by using site-directed mutagenesis. Both wild-type papain and an Asp158 Asn variant were produced in a baculovirus-insect cell expression system, purified to homogeneity from the culture, and characterized kinetically. With CBZ-Phe-Arg-MCA as substrate, the kcat/KM and kcat values...

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PMID: 2397208
DOI: 10.1021/bi00480a021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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