Article
Reconstruction of 3D stacked-up structures by rat small hepatocytes on microporous membranes.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology - 1 Oct 2005
Sudo Ryo, Mitaka Toshihiro, Ikeda Mariko, Tanishita Kazuo
Abstract excerpt
The three-dimensional (3D) culture of hepatocytes is essential for the reconstruction of functional hepatic tissues in vitro. In the present experiment, we developed a 3D-culture method in order to reconstruct hepatic cordlike structures by stacking up two-dimensional (2D) tissues composed of rat small hepatocytes (SHs), which are hepatic progenitor cells. Pairs of membranes were prepared and the cells were...
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