Article
Fluorescence studies of the binding of bacteriophage M13 gene V mutant proteins to polynucleotides.
European journal of biochemistry - 15 Jun 1992
Stassen A P, Harmsen B J, Schoenmakers J G, Hilbers C W, Konings R N
Abstract excerpt
This investigation describes how the binding characteristics of the single-stranded DNA-binding protein encoded by gene V of bacteriophage M13, are affected by single-site amino acid substitutions. The series of mutant proteins tested includes mutations in the purported monomer-monomer interaction region as well as mutations in the DNA-binding domain at positions which are thought to be functionally involved in...
Topics
- Bacteriophages
- Binding Sites
- DNA-Binding Proteins
- Genes, Viral
- Magnetic Resonance Spectroscopy
- Mutation
- Polydeoxyribonucleotides
- Protein Conformation
- Solubility
- Spectrometry, Fluorescence
- Viral Proteins