Article
Independent tyrosyl contributions to the CD of Ff gene 5 protein and the distinctive effects of Y41H and Y41F mutants on protein-protein cooperative interactions.
Protein science : a publication of the Protein Society - 1 Mar 2002
Mou Tung-Chung, Sreerama Narasimha, Terwilliger Thomas C, Woody Robert W, Gray Donald M
Abstract excerpt
The gene 5 protein (g5p) of the Ff virus contains five Tyr, individual mutants of which have now all been characterized by CD spectroscopy. The protein has a dominant tyrosyl 229-nm L(a) CD band that is shown to be approximately the sum of the five individual Tyr contributions. Tyr41 is particularly important in contributing to the high cooperativity with which the g5p binds to ssDNA, and Y41F and Y41H mutants...
Topics
- Amino Acid Substitution
- Circular Dichroism
- DNA, Single-Stranded
- DNA-Binding Proteins
- Dimerization
- Escherichia coli
- Mutation
- Protein Binding
- Protein Structure, Secondary
- Recombinant Proteins