Article
Subdomain-specific collapse of denatured staphylococcal nuclease revealed by single molecule fluorescence resonance energy transfer measurements.
The journal of physical chemistry. B - 3 Sept 2009
Liu Pengcheng, Meng Xianglan, Qu Peng, Zhao Xin Sheng, Wang Chih-chen
Abstract excerpt
By using single molecule fluorescence resonance energy transfer (smFRET), the equilibrium denaturation of staphylococcal nuclease (SNase) induced by guanidinium hydrochloride (GdmCl) has been investigated. We have characterized the collapse of the denatured chain and its relation to structure formation. Two mutants, K28C/H124C and K28C/K97C, were constructed and labeled for monitoring the behaviors of the global...
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