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Article

Heterologous gene expression using self-assembled supra-molecules with high affinity for HSP70 chaperone.

Nucleic acids research - 1 Jan 2005

Ahn Ji-Young, Choi Hyung, Kim Yang-Hoon, Han Kyung-Yeon, Park Jin-Seung, Han Sung-Sik, Lee Jeewon

Abstract excerpt

Contrary to the results of direct expression, various human proteins (ferritin light-chain, epithermal growth factor, interleukin-2, prepro-ghrelin, deletion mutants of glutamate decarboxylase and arginine deiminase, and mini-proinsulin) were all soluble in Escherichia coli cytoplasm when expressed with the N-terminus fusion of ferritin heavy-chain (FTN-H). Through systematic investigations, we have found that a...

Topics

Amino Acid Motifs
Amino Acid Sequence
Artificial Gene Fusion
Binding Sites
Escherichia coli Proteins
Ferritins
Gene Expression
Genetic Vectors
HSP70 Heat-Shock Proteins
Humans
Microscopy, Electron, Transmission

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Recombinant Fusion Proteins

Sequence Alignment

Source

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Identifiers

PMID: 16006621
DOI: 10.1093/nar/gki692
PMCID: PMC1174908

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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