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Article

Characterization of monoclonal antibodies against altered (T103I) amidase from Pseudomonas aeruginosa.

Molecular biotechnology - 1 Jul 2005

Martins Sónia, Karmali Amin, Andrade Jorge, Custódio Ana, Serralheiro Maria Luísa

Abstract excerpt

Monoclonal antibodies (MAbs) against mutant (T103I) amidase from Pseudomonas aeruginosa were raised by hybridoma technology. To select MAbs suitable for immunoaffinity chromatography, hybridoma clones secreting polyol-responsive MAbs (PR-MAbs) were screened that bind antigen tightly but release under mild and nondenaturing elution conditions. It was found that about 10% of enzyme-linked immunosorbent assay...

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Identifiers

PMID: 15988046
DOI: 10.1385/MB:30:3:207

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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