Engineering a regulatable enzyme for homogeneous immunoassays.

doi:10.1038/5243

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Article

Engineering a regulatable enzyme for homogeneous immunoassays.

Nature biotechnology - 1 Jan 1999

Legendre D, Soumillion P, Fastrez J

Abstract excerpt

We have engineered the phage displayed TEM-1 beta-lactamase to generate enzymes that can be used in homogeneous immunoassays because their activity can be modulated by binding to monoclonal antibodies (Mabs) raised against an unrelated protein. Random peptide libraries were genetically inserted i...

Topics

Antibodies, Monoclonal
Bacteriophages
Base Sequence
Binding Sites
Capsid Proteins
DNA-Binding Proteins
Enzymes
Epitopes
Immunoassay
Molecular Sequence Data
Mutation
Peptide Library
Prostate-Specific Antigen
Protein Engineering
Recombinant Proteins
Selection, Genetic
Streptavidin
Viral Fusion Proteins

Source

Open in PubMed

Identifiers

PMID: 9920272
DOI: 10.1038/5243

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9920272-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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