Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Engineering a regulatable enzyme for homogeneous immunoassays.

Nature biotechnology - 1 Jan 1999

Legendre D, Soumillion P, Fastrez J

Abstract excerpt

We have engineered the phage displayed TEM-1 beta-lactamase to generate enzymes that can be used in homogeneous immunoassays because their activity can be modulated by binding to monoclonal antibodies (Mabs) raised against an unrelated protein. Random peptide libraries were genetically inserted into three loops to create hybrid enzymes with binding sites for Mabs. Insertion points were chosen to be close enough...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antibodies, Monoclonal
Share to Base Sequence
Share to Binding Sites

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA-Binding Proteins

Share to Molecular Sequence Data

Share to Mutation

Share to Recombinant Proteins

Share to Selection, Genetic

Source

Identifiers

PMID: 9920272
DOI: 10.1038/5243

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Engineering a regulatable enzyme for homogeneous immunoassays.PMID 9920272Read ↗

TEM-1 beta-lactamase as a scaffold for protein recognition and assay.Semantic proximity Read ↗

Tailoring molecular sensing for peptide displaying engineered enzymesSemantic proximity Read ↗

Design and engineering of genetically encoded protein biosensors for small moleculesSemantic proximity Read ↗

Selection of metalloenzymes by catalytic activity using phage display and catalytic elution.Semantic proximity Read ↗

Design of Protein-Based Biosensors for Selective Detection of Benzene Groups of Pollutants.Semantic proximity Read ↗

Development of homogeneous immunoassays based on protein fragment complementation.Semantic proximity Read ↗

A molecular sensor system based on genetically engineered alkaline phosphatase.Semantic proximity Read ↗

Engineering allosteric regulation into the hinge region of a circularly permuted TEM-1 beta-lactamase.Semantic proximity Read ↗

Transcriptional regulators à la carte: engineering new effector specificities in bacterial regulatory proteins.Semantic proximity Read ↗

Selection of catalytically active biotin ligase and trypsin mutants by phage display.Semantic proximity Read ↗

Determination and analysis of antigenic epitopes of prostate specific antigen (PSA) and human glandular kallikrein 2 (hK2) using synthetic peptides and computer modelingSemantic proximity Read ↗

Construction of a biosensor mutant of Comamonas testosteroni for testosterone determination by cloning the EGFP gene downstream to the regulatory region of the 3,17β-HSD gene.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.