Arg-188 and Trp-144 are implicated in the binding of urea and acetamide to the active site of the amidase from Pseudomonas aeruginosa.

doi:10.1016/0167-4838(94)90102-3

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Article

Arg-188 and Trp-144 are implicated in the binding of urea and acetamide to the active site of the amidase from Pseudomonas aeruginosa.

Biochimica et biophysica acta - 16 Mar 1994

Tata R, Marsh P, Brown P R

Abstract excerpt

Urea is a time-dependent active-site-directed inhibitor of Pseudomonas aeruginosa amidase. We found that 20 mM hydroxylamine caused bound urea to be released from the inactive urea:amidase complex with the restoration of enzyme activity. Bound urea restricts the titrability of the enzyme's -SH gr...

Topics

Acetamides
Amidohydrolases
Arginine
Base Sequence
Binding Sites
Dithionitrobenzoic Acid
Enzyme Stability
Hot Temperature
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Pseudomonas aeruginosa
Tryptophan
Urea

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