One-step affinity purification of amidase from mutant strains of Pseudomonas aeruginosa.

doi:10.1016/0300-9084(89)90021-7

Back to search

Article

One-step affinity purification of amidase from mutant strains of Pseudomonas aeruginosa.

Biochimie - 1 Jan 2000

Domingos A, Karmali A, Brown P R

Abstract excerpt

Amidases (acylamide amidohydrolase EC 3.5.1.4) from mutant strains (i.e., B6, AI3, AIU1N, OUCH 4 and L10) of Pseudomonas aeruginosa were purified in one-step by ligand affinity chromatography using Epoxy-activated Sepharose 4B-acetamide. The yields of the purified enzymes were about 90% for all m...

Topics

Acetamides
Amidohydrolases
Chromatography, Affinity
Chromatography, Gel
Epoxy Resins
Molecular Weight
Mutation
Pseudomonas aeruginosa
Sepharose
Urea

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.